Local integrands for the five-point amplitude in planar N=4 SYM up to five loops

Integrands for colour ordered scattering amplitudes in planar N=4 SYM are dual to those of correlation functions of the energy-momentum multiplet of the theory. The construction can relate amplitudes with different numbers of legs. By graph theory methods the integrand of the four-point function of energy-momentum multiplets has been constructed up to six loops in previous work. In this article we extend this analysis to seven loops and use it to construct the full integrand of the five-point amplitude up to five loops, and in the parity even sector to six loops. All results, both parity even and parity odd, are obtained in a concise local form in dual momentum space and can be displayed efficiently through graphs. We have verified agreement with other local formulae both in terms of supertwistors and scalar momentum integrals as well as BCJ forms where those exist in the literature, i.e. up to three loops. Finally we note that the four-point correlation function can be extracted directly from the four-point amplitude and so this uncovers a direct link from four- to five-point amplitudes.Comment: 29 pages LaTeX, 8 figure

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Between but not within species variation in the distribution of fitness effects

New mutations provide the raw material for evolution and adaptation. The distribution of fitness effects (DFE) describes the spectrum of effects of new mutations that can occur along a genome, and is therefore of vital interest in evolutionary biology. Recent work has uncovered striking similarities in the DFE between closely related species, prompting us to ask whether there is variation in the DFE among populations of the same species, or among species with different degrees of divergence, i.e., whether there is variation in the DFE at different levels of evolution. Using exome capture data from six tree species sampled across Europe we characterised the DFE for multiple species, and for each species, multiple populations, and investigated the factors potentially influencing the DFE, such as demography, population divergence and genetic background. We find statistical support for there being variation in the DFE at the species level, even among relatively closely related species. However, we find very little difference at the population level, suggesting that differences in the DFE are primarily driven by deep features of species biology, and that evolutionarily recent events, such as demographic changes and local adaptation, have little impact

Local integrands for the five-point amplitude in planar N=4 SYM up to five loops

Integrands for colour ordered scattering amplitudes in planar N=4 SYM are dual to those of correlation functions of the energy-momentum multiplet of the theory. The construction can relate amplitudes with different numbers of legs. By graph theory methods the integrand of the four-point function of energy-momentum multiplets has been constructed up to six loops in previous work. In this article we extend this analysis to seven loops and use it to construct the full integrand of the five-point amplitude up to five loops, and in the parity even sector to six loops. All results, both parity even and parity odd, are obtained in a concise local form in dual momentum space and can be displayed efficiently through graphs. We have verified agreement with other local formulae both in terms of supertwistors and scalar momentum integrals as well as BCJ forms where those exist in the literature, i.e. up to three loops. Finally we note that the four-point correlation function can be extracted directly from the four-point amplitude and so this uncovers a direct link from four- to five-point amplitudes

High Amorphous Vinyl Alcohol-Silica Bionanocomposites: Tuning Interface Interactions with Ionic Liquids

Herein, we demonstrate the effect of imidazolium ionic liquids (IL) applied as additives in the <i>in situ</i> formation of high amorphous vinyl alcohol (HAVOH)-silica bionanocomposites, using a simple sol–gel process approach. A complementary set of alkyl-, ether-, and carboxy-functionalized IL was used, allowing silica structure control and polymer–silica interphase tuning. Consequently, hybrids with diverse morphologies, as well as improved thermo-mechanical and barrier properties, were obtained. This diversity also highlighted the systems’ dependency on the IL’s molecular structure, where both the cation and anion influenced the hybrids’ final properties. This could be evidenced as the polar group functionalized-IL (ether- and carboxy-functionalized IL) allowed the formation of multiple hydrogen bonds at the organic–inorganic interphase, inducing a fine hybrid morphology with well-dispersed silica nanodomains. This significantly increased the storage (∼50%) and tensile moduli (∼20%), extensibility (up to 300%), and glass transition temperature (>20 °C) and decreased the water vapor permeability (∼50%), which are desirable characteristics for potential food and medical packaging

Antimicrobial Evaluation of Diterpenes from Copaifera langsdorffii Oleoresin Against Periodontal Anaerobic Bacteria

The antimicrobial activity of four labdane-type diterpenes isolated from the oleoresin of Copaifera langsdorffii as well as of two commercially available diterpenes (sclareol and manool) was investigated against a representative panel of microorganisms responsible for periodontitis. Among all the evaluated compounds, (−)-copalic acid (CA) was the most active, displaying a very promising MIC value (3.1 µg mL−1; 10.2 µM) against the key pathogen (Porphyromonas gingivalis) involved in this infectious disease. Moreover, CA did not exhibit cytotoxicity when tested in human fibroblasts. Time-kill curve assays performed with CA against P. gingivalis revealed that this compound only inhibited the growth of the inoculums in the first 12 h (bacteriostatic effect). However, its bactericidal effect was clearly noted thereafter (between 12 and 24 h). It was also possible to verify an additive effect when CA and chlorhexidine dihydrochloride (CHD, positive control) were associated at their MBC values. The time curve profile resulting from this combination showed that this association needed only six hours for the bactericidal effect to be noted. In summary, CA has shown to be an important metabolite for the control of periodontal diseases. Moreover, the use of standardized extracts based on copaiba oleoresin with high CA contents can be an important strategy in the development of novel oral care products

Fungal Transformation and Schistosomicidal Effects of Pimaradienoic Acid

The schistosomicidal effects of pimaradienoic acid (PA) and two derivatives, obtained by fungal transformation in the presence of Aspergillus ochraceus, were investigated. PA was the only compound with antischistosomal activity among the three diterpenes studied, with the ability to significantly reduce the viability of the parasites at concentrations ranging from 25 to 100 mu M. PA also promoted morphological alterations of the tegument of Schistosoma mansoni, separated all the worm couples, and affected the production and development of eggs. Moreover, this compound was devoid of toxicity toward human fibroblasts. In a preliminary in vivo experiment, PA at a dose of 100 mg/kg significantly diminished the number of parasites in infected Balb/c mice. Taken together, these results show that PA may be potentially employed in the discovery of novel schistosomicidal agents, and that diterpenes are an important class of natural compounds for the investigation of agents capable of fighting the parasite responsible for human schistosomiasis.FAPESPFAPESP [2007/54762-8 an 2008/07696-2]CNPqCNP

The HiggsTools handbook: a beginners guide to decoding the Higgs sector

This report summarises some of the activities of the HiggsTools initial training network working group in the period 2015–2017. The main goal of this working group was to produce a document discussing various aspects of state-of-the-art Higgs physics at the large hadron collider (LHC) in a pedagogic manner. The first part of the report is devoted to a description of phenomenological searches for new physics (NP) at the LHC. All of the available studies of the couplings of the new resonance discovered in 2012 by the ATLAS and CMS experiments (Aad et al (ATLAS Collaboration) 2012 Phys. Lett. B 716 1–29; Chatrchyan et al (CMS Collaboration) 2012 Phys. Lett. B 716 30–61) conclude that it is compatible with the Higgs boson of the standard model (SM) within present precision. So far the LHC experiments have given no direct evidence for any physical phenomena that cannot be described by the SM. As the experimental measurements become more and more precise, there is a pressing need for a consistent framework in which deviations from the SM predictions can be computed precisely. Such a framework should be applicable to measurements in all sectors of particle physics, not only LHC Higgs measurements but also electroweak precision data, etc. We critically review the use of the κ-framework, fiducial and simplified template cross sections, effective field theories, pseudoobservables and phenomenological Lagrangians. Some of the concepts presented here are well known and were used already at the time of the large electron–positron collider (LEP) experiment. However, after years of theoretical and experimental development, these techniques have been refined, and we describe new tools that have been introduced in order to improve the comparison between theory and experimental data. In the second part of the report, we propose $\phi^*_{\eta}$ as a new and complementary observable for studying Higgs boson production at large transverse momentum in the case where the Higgs boson decays to two photons. The $\phi^*_{\eta}$ variable depends on measurements of the angular directions and rapidities of the two Higgs decay products rather than the energies, and exploits the information provided by the calorimeter in the detector. We show that, even without tracking information, the experimental resolution for $\phi^*_{\eta}$ is better than that of the transverse momentum of the photon pair, particularly at low transverse momentum. We make a detailed study of the phenomenology of the $\phi^*_{\eta}$ variable, contrasting the behaviour with the Higgs transverse momentum distribution using a variety of theoretical tools including event generators and fixed order perturbative computations. We consider the theoretical uncertainties associated with both $p_{TH}$ and $\phi^*_{\eta}$ distributions. Unlike the transverse momentum distribution, the distribution is well predicted using the Higgs effective field theory in which the top quark is integrated out—even at large values of $\phi^*_{\eta}$ —thereby making this a better observable for extracting the parameters of the Higgs interaction. In contrast, the potential of the $\phi^*_{\eta}$ distribution as a probe of NP is rather limited, since although the overall rate is affected by the presence of additional heavy fields, the shape of the $\phi^*_{\eta}$ distribution is relatively insensitive to heavy particle thresholds